Handheld Aptamer-Magnetic Bead-Quantum Dot Sensor for Rickettsiae

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-10-C-0051
Agency Tracking Number: A093-152-0731
Amount: $119,978.00
Phase: Phase I
Program: SBIR
Awards Year: 2010
Solicitation Year: 2009
Solicitation Topic Code: A09-152
Solicitation Number: 2009.3
Small Business Information
4100 N. W. Loop 410Suite 230, San Antonio, TX, 78229
DUNS: 000000000
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 John Bruno
 Senior Scientist
 (210) 731-0000
 john.bruno@otcorp.com
Business Contact
 William Henderson
Title: Chief Financial Officer
Phone: (210) 731-0000
Email: contractsmgr@creare.com
Research Institution
N/A
Abstract
Operational Technologies (OpTech) has developed a rapid (15 minute) ultrasensitive one-step plastic-adherent DNA aptamer-magnetic bead (MB) plus aptamer-quantum dot (QD) sandwich assay for Campylobacter detection (J. Fluorescence 19:427-435, 2009), which is quantified with a commercially available handheld and battery-operated fluorometer. This highly portable and facile system has a validated limit of detection of < 10 cfu/ml for C. jejuni and works with push-button ease under rugged field conditions. Therefore, OpTech proposes extension of its patent-pending one-step plastic cuvette-adherent aptamer-MB-aptamer-QD sandwich assays for sensitive antigen (not antibody) detection of early pre-symptomatic rickettsial targets (from the spotted fever and typhus groups) using its lightweight and rugged handheld fluorometer. OpTech will obtain killed rickettsiae from the Army and develop a target cocktail for DNA aptamer selection to enable a general rickettsial assay. At the end of Phase I, OpTech will deliver 100 lyophilized prototype rickettsial assays to the Army for independent testing and evaluation. In Phase II, OpTech will optimize assay performance with respect to specificity, sensitivity and shelf life. OpTech will also optimize shelf life to > 2 years at 35oC by increasing the level of trehalose or other excipients and innovative vacuum or air-tight packaging of the lyophilized assays.

* Information listed above is at the time of submission. *

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