Handheld Aptamer-Magnetic Bead-Quantum Dot Sensor for Rickettsiae

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$69,982.00
Award Year:
2010
Program:
SBIR
Phase:
Phase I
Contract:
W81XWH-10-C-0051
Award Id:
97696
Agency Tracking Number:
A093-152-0731
Solicitation Year:
n/a
Solicitation Topic Code:
Army 09-152
Solicitation Number:
n/a
Small Business Information
4100 N. W. Loop 410, Suite 230, San Antonio, TX, 78229
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
154088710
Principal Investigator:
JohnBruno
Senior Scientist
(210) 731-0000
john.bruno@otcorp.com
Business Contact:
WilliamHenderson
Chief Financial Officer
(210) 731-0000
contractsmgr@creare.com
Research Institute:
n/a
Abstract
Operational Technologies (OpTech) has developed a rapid (15 minute) ultrasensitive one-step plastic-adherent DNA aptamer-magnetic bead (MB) plus aptamer-quantum dot (QD) sandwich assay for Campylobacter detection (J. Fluorescence 19:427-435, 2009), which is quantified with a commercially available handheld and battery-operated fluorometer. This highly portable and facile system has a validated limit of detection of < 10 cfu/ml for C. jejuni and works with push-button ease under rugged field conditions. Therefore, OpTech proposes extension of its patent-pending one-step plastic cuvette-adherent aptamer-MB-aptamer-QD sandwich assays for sensitive antigen (not antibody) detection of early pre-symptomatic rickettsial targets (from the spotted fever and typhus groups) using its lightweight and rugged handheld fluorometer. OpTech will obtain killed rickettsiae from the Army and develop a target cocktail for DNA aptamer selection to enable a general rickettsial assay. At the end of Phase I, OpTech will deliver 100 lyophilized prototype rickettsial assays to the Army for independent testing and evaluation. In Phase II, OpTech will optimize assay performance with respect to specificity, sensitivity and shelf life. OpTech will also optimize shelf life to > 2 years at 35oC by increasing the level of trehalose or other excipients and innovative vacuum or air-tight packaging of the lyophilized assays.

* information listed above is at the time of submission.

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