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INNOVATIVE TECHNOLOGY FOR GENE EXPRESSION STUDIES

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 1R43CA094361-01
Agency Tracking Number: CA094361
Amount: $359,546.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 2002
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
ALDERON BIOSCIENCES, INC. 2810 MERIDIAN PKY, STE 152
DURHAM, NC 27713
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 ROBERT HENKENS
 (919) 544-8220
 HENKENS@EARTHLINK.NET
Business Contact
 JOHN O'DALY
Phone: (919) 544-8220
Email: HENKENS@EARTHLINK.NET
Research Institution
N/A
Abstract

Alderon Biosciences proposes to develop a new electrochemical method for detection of specific nucleic acid sequences without enzymatic, fluorescent or radioactive labels and to show the feasibility of applying this method in an electrochemical plate (E-Plate) format to studies of gene expression in cancer. The E-Plate, an electrical analog of a traditional microplate and reader, will be developed as a tool to identify, quantify and study mRNAs and cDNA libraries of specific interest for cancer studies. Oligonucleotide probes containing electroactive bases will be used to capture the target sequences by hybridization on separately addressable elements of the E-Plate. Probe-target hybridization generates a signal in the presence of a buffer containing a mediator. The signal can be read out rapidly and be used to evaluate differential gene expression patterns. The expected result is an innovative electrochemical plate and reader that provides researchers with a rapid, facile and affordable method to probe small samples for specific mRNA or cDNA sequences. Another distinctive feature of the proposed approach that will be demonstrated in studies with human tumor suppressor mRNA targets is the ability to recover captured targets from the wells of the E-Plate for subsequent cloning, sequencing, or mass spectrometric analysis.

* Information listed above is at the time of submission. *

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