SBIR Phase II: Detection and Identification Instrument for Single Molecule Analysis

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: 0450539
Agency Tracking Number: 0319204
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2003
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
44 Hunt Street, Watertown, MA, 02472
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Arieh Karger
 (617) 668-6827
Business Contact
 Gerald Entine
Title: Mr
Phone: (617) 926-1167
Research Institution
This Small Business Innovation Research (SBIR) Phase II project proposes to develop a novel, low cost laboratory instrument for genetic analysis and single molecule studies. The technology is suitable for the detection and identification of DNA and RNA through fluorescent hybridization probes without the need for Polymerase Chain Reaction (PCR) amplification, or for proteins and small molecules through fluorescence immunoassays. The general scheme is based on single molecule detection (SMD) and utilizes the two-color cross-correlation spectroscopy (TC-FCCS) technique with coincident detection analysis scheme to simultaneously probe ten focal regions of a microfluidic assay. High efficiency single photon detectivity Geiger mode microavalanche photodiode (uAPD) arrays will function as detection elements. The commercial application of this project will be on biological and medical research, and on the drug development process. Examples of potential applications range from the study of conformational dynamics and interactions of macromolecules to biochemical kinetics of single molecules.

* Information listed above is at the time of submission. *

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