Rapid Generation of High-affinity Aptamers for Quantification of Protein Biomarkers

Award Information
Agency: Department of Defense
Branch: Army
Contract: W81XWH-08-C-0012
Agency Tracking Number: A072-147-1286
Amount: $70,072.00
Phase: Phase I
Program: SBIR
Awards Year: 2007
Solicitation Year: 2007
Solicitation Topic Code: A07-147
Solicitation Number: 2007.2
Small Business Information
44 Hunt Street, Watertown, MA, 02472
DUNS: 073804411
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Daniel Hall
 Staff Scientist
 (617) 668-6822
Business Contact
 Gerald Entine
Title: President
Phone: (617) 668-6800
Email: GEntine@rmdinc.com
Research Institution
The generation of genomic and proteomic data with the goal of biomarker discovery for disease diagnostics or prognosis is a powerful approach. However, biomarkers identified from a typically small sample size must be validated on a large population. A limiting factor is the availability of high-quality reagents for the quantification of the biomarkers in a complex medium such as serum. The assay must be technically simple and amenable to high-throughput procedures. Screening of recombinant antibody libraries is a powerful method for the generation of affinity reagents. However, “affinity maturation” techniques required to produce high affinity antibodies is a relatively slow procedure as it requires library isolation, diversification, and retransformation back into cells. Radiation Monitoring Devices, Inc. proposes the high-throughput generation of nucleic acids-based aptamer affinity reagents. We introduce a novel technique for generating aptamers with high affinity and specificity. In addition, we will utilize a screening technique that does not require sample modification with labels or separation of the bound/unbound aptamers. This will increase the number of clones for which we are able to obtain binding data. Finally, because selection occurs outside of cells, the entire procedure is rapid including all maturation steps for improving the binding characteristics.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government