Single Molecule Instrument for Surface Enhanced Raman Optical Activity of Biomolecules

Award Information
Agency: National Aeronautics and Space Administration
Branch: N/A
Contract: NNA07BA45C
Agency Tracking Number: 053920
Amount: $559,992.00
Phase: Phase II
Program: SBIR
Awards Year: 2007
Solicitation Year: 2005
Solicitation Topic Code: S2.05
Solicitation Number: N/A
Small Business Information
44 Hunt Street, Watertown, MA, 02472-4699
DUNS: 073804411
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Rajan Gurjar
 Principal Investigator
 (617) 668-6801
Business Contact
 Nancy Marshall
Title: Business Official
Phone: (617) 668-6801
Research Institution
Stereochemistry is an essential element of our organic life. Only certain enantiomers are useful as drugs for the human body. Raman optical activity (ROA) provides stereochemical information down to the bond levels. Many biomolecules like proteins and DNA can be studied to understand their structural chemistry and structure related dynamics. These methods do not require material in the crystalline form and hence can be very useful tools. However, ROA signals are even weaker than the Raman signals. Using an important biomolecule, we have demonstrated in Phase I that ROA can be enhanced using nanoparticles. Not only did the ROA ratio increase by two orders of magnitude, the measurement time reduced from several hours to 10 seconds. Phase II work will focus on enhancing ROA signals in different subspecies of biomolecules, namely amino acids and proteins, and developing the appropriate colloidal chemistry. Use of nanoparticles is known to enhance Raman signals by several orders of magnitude. Our goal is to achieve similar gains in ROA signals by using a sensitive detection system in combination with improved surface enhanced chemistry and microfluidics-based single-molecule detection techniques. This will result in improved precision of measurement and shorten measurement time.

* Information listed above is at the time of submission. *

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