SBIR Phase I: A Protein Probe for High-Throughput SNP Screening

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: 0712121
Agency Tracking Number: 0712121
Amount: $99,994.00
Phase: Phase I
Program: SBIR
Awards Year: 2007
Solicitation Year: 2006
Solicitation Topic Code: BT
Solicitation Number: NSF 06-598
Small Business Information
Radiation Monitoring Devices Inc
44 Hunt Street, Ste 2100-162, Watertown, MA, 02472
DUNS: 073804411
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Daniel Hall
 DPhil
 (617) 668-6801
 DHall@RMDInc.com
Business Contact
 Daniel Hall
Title: DPhil
Phone: (617) 668-6801
Email: DHall@RMDInc.com
Research Institution
N/A
Abstract
This Small Business Small Business Innovation Research (SBIR) Phase I research project aims to develop a reagent that binds specifically to DNA mismatches for use in a microarray-based single nucleotide polymorphism (SNP) assay. Directed evolution will be used to isolate variants of a DNA repair protein with substrate binding properties optimized for this assay. Key challenges will be decreasing the affinity for perfectly complementary oligonucleotides and increasing the spectrum of mismatches bound with sufficient specificity. Because this assay does not rely on subtle differences in hybridization energy for detection of mismatch-containing duplexes, the microarrays require only a small number of probes per SNP (2-4) and are not limited to using short probes. The availability of an inexpensive, flexible, and high throughput SNP detection methodology could lead to better understanding of the genetic components of complex diseases and advance the development of personalized medicine where disease prognosis and treatment is individually tailored.

* information listed above is at the time of submission.

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