Generation of bivalent aptamers with high affinity and selectivity for detection of viruses and bacteria from environmental samples

Award Information
Agency: Department of Homeland Security
Branch: N/A
Contract: NBCHC090038
Agency Tracking Number: 0911090
Amount: $99,999.00
Phase: Phase I
Program: SBIR
Awards Year: 2009
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
44 Hunt Street, Watertown, MA, 02472
DUNS: 073804411
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Daniel Hall
 (617) 668-6800
 DHall@RMDInc.com
Business Contact
 Gerald Entine
Phone: (617) 668-6801
Email: GEntine@RMDInc.com
Research Institution
N/A
Abstract

Better methodologies for the rapid detection of pathogenic bacteria and viruses are needed. A problem with current antibody-based assays is cross-reactivity with similar, but harmless, species. RMD, Inc., proposes developing nucleic acid-based affinity reagents (aptamers) against the spore coat of the model organism Bacillus thuringiensis. Aptamers displaying fast binding rates (kon) will be selected in order to increase the speed of the assay. A novel approach using bivalent, bispecific aptamers will be utilized to increase the binding affinities to at least equal that of typical antibodies. The selectivity of the aptamers will be enhanced by employing counterselection procedures whereby aptamers binding related species are deselected. Finally, the aptamers will be applied to an ELISA-like protocol and their performance directly compared to antibodies. Commercial Applications: A more rapid, sensitive, and selective assay for the detection of bacteria and viruses will have widespread applications in homeland security, the military, healthcare, and food safety. The assay could be used to screen ports of entry, public places of interest, military sites and battlefields for evidence of biological threat agents. Likewise, the assay could be used in a clinical setting, in a laboratory or at the point-of-care, to rapidly diagnose infectious diseases.

* Information listed above is at the time of submission. *

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