A HEPATOCYTE SPHEROID BIOASSAY SYSTEM FOR DRUG DISCOVERY

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43DK059109-01A2
Agency Tracking Number: DK059109
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
ALGENIX, INC., 5264 OXFORD ST N, SHOREVIEW, MN, 55126
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 LEONARD RUIZ
 (651) 490-0212
 LPRUIZ@ATTBI.NET
Business Contact
 LEONARD RUIZ
Phone: (651) 490-0212
Email: LPRUIZ@MN.MEDIAONE.NET
Research Institution
N/A
Abstract
Description (provided by applicant): The liver is the most important organ in metabolizing xenobiotics and drugs. Liver cells (hepatocytes) are used frequently in testing drug metabolism and hepatotoxicity. Hepatocytes in culture can form spheroids that exhibit higher liver-specific functions and sustain high viability longer in culture. This proposed research is to develop an integrated system employing hepatocyte spheroids for both screening purposes and for metabolic studies. In phase I studies, we will develop a high throughput screening system suitable for integration with a miniaturized bioartificial liver for such studies. We will also demonstrate genetic metabolic engineering of hepatocyte spheroids. The analysis of the results will be facilitated by the use of a cDNA microarray immobilized with most relevant DNA sequences. The proposed work will fill a critical need for bioassay systems for high throughput screening.

* Information listed above is at the time of submission. *

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