Advanced Platform of RNAi Technology

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$99,510.00
Award Year:
2003
Program:
SBIR
Phase:
Phase I
Contract:
1R43GM069369-01
Award Id:
66496
Agency Tracking Number:
GM069369
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
ALLELE BIOTECH & PHARMACEUTICALS, 6827 NANCY RIDGE DR, SAN DIEGO, CA, 92121
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
JIWU WANG
(858) 587-6645
JIWUWANG@ALLELEBIOTECH.COM
Business Contact:
LIN WANG
(858) 337-4718
LINWANG@ALLELEBIOTECH.COM
Research Institution:
n/a
Abstract
RNA interference (RNAi) is a powerful tool for gene function studies and drug development. Currently, application of this technology is largely limited by the efficiency of delivering RNAi-inducing reagents into mammalian cells. The aim of this proposal is to establish techniques to efficiently transfect siRNA-expressing cassettes. We have already developed a system to induce R NAi effects in mammalian I cells using novel DNA-based expression cassettes that offer gr eat advantages over other RNA or plasmid-based methods. We plan to take advantage of the unique design of our existing core technology by using transducing peptides as dell very vehicles. This will be particularly useful for RNAi-based gene silencing in traditionally hard to transfect cells such as primary cultures. T hese techniques can be further integrated into novel platforms for high throughput discovery and adapted for use in live animals. Development of these methods may also lay the foundations for exciting applications of our RNAi technology as therapeutic agents.

* information listed above is at the time of submission.

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