T-cell receptor based therapeutics for cytomegalovirus

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43AI056782-01
Agency Tracking Number: AI056782
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2003
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 (954) 443-8600
Business Contact
Phone: (954) 443-8600
Research Institution
DESCRIPTION (provided by investigator): The goal of these studies is to evaluate the feasibility of using a T-cell receptor based fusion protein as a targeting agent for therapies for CMV, which remains a major cause of morbidity and mortality inimmunocompromised patients. For these studies a TCR recognizing a distinct, naturally processed, highly immunogenic peptide fragment from the human cytomegalovirus tegument protein pp65 presented on the cell surface in the context of HLA-A2 was cloned. This TCR was isolated from CMV peptide specific CD8+ T-cells that were generated by CMV peptide stimulation of HLA-A2 positive human PBMC. A CMV specific single chain TCR/IgG1 fusion protein, CMVscTCR/IgG1, was prepared and will be expressed at high levels in mammalian cells and purified for characterization. The fusion protein will be characterized in vitro for MHC restricted peptide specific binding, stability, affinity, ability to target antigen positive cells, and ability to conjugate target and effector cells. Finally, the CMVscTCR/IgG1 fusion protein will be evaluated in vivo for pharmacokinetics and toxicity. Successful completion of these studies will lead to in vivo efficacy studies in a novel mouse model of human CMV infection and to eventual production of TCR-based targeted CMV therapeutics for commercialization.

* Information listed above is at the time of submission. *

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