Microfluidic Dielectrophoretic Screening System

Award Information
Agency:
Department of Defense
Branch
Office for Chemical and Biological Defense
Amount:
$69,994.00
Award Year:
2010
Program:
SBIR
Phase:
Phase I
Contract:
W81XWH-10-C-0217
Award Id:
96638
Agency Tracking Number:
C101-107-0024
Solicitation Year:
n/a
Solicitation Topic Code:
CBD 10-107
Solicitation Number:
n/a
Small Business Information
Photonic Systems Division, 20600 Gramercy Place, Bldg. 100, Torrance, CA, 90501
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
153865951
Principal Investigator:
Gregory Zeltser
Principal Scientist
(310) 320-3088
psproposals@poc.com
Business Contact:
Gordon Drew
Chief Financial Officer
(310) 320-3088
gedrew@poc.com
Research Institution:
n/a
Abstract
To address the U.S. Army/DTRA's CBD program need for a robust screening process for use with large panels of enzymes to identify variants with higher affinity for organophosphorus (OP) substrates, Physical Optics Corporation (POC) proposes to develop a Microfluidic Dielectrophoretic Screening (MDS) system. This proposed system is based on the new design of a microfluidic mixer, enzymatic reactor, and readout unit that uses in-house developed mature components. The innovations in the MDS system will enable the completion of enzyme kinetic measurements in 15 minutes. Also, the MDS system requires nanogram quantities of an enzyme; and the microfluidic chip is self-contained, reusable, and sufficiently robust to withstand routine cleaning with highly basic and alcoholic solutions. As a result, this technology will provide the DoD with an improved capability to develop novel scavengers of OP nerve agents. In Phase I, POC will demonstrate the feasibility of the MDS system by developing a robust and high-throughput prototype capable of identifying enzyme variants with reduced KM values for OP compounds. In Phase II, POC plans to further develop, refine, and validate the MDS system, using nerve agent simulants followed by validation with OP nerve agents, at a testing facility approved for using these materials.

* information listed above is at the time of submission.

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