PHOTOCLEAVABLE REAGENTS FOR ISOLATION OF STEM CELLS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,000.00
Award Year:
1997
Program:
SBIR
Phase:
Phase I
Contract:
AI40753-01
Agency Tracking Number:
39320
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Ambergen, Inc.
1106 COMMONWEALTH AVE, Boston, MA, 02215
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
OLEJNIK, JERZY
() -
Business Contact:
(617) 277-2547
Research Institution:
n/a
Abstract
The isolation of stem cells is a critical technology for the treatment of a wide range of diseases. Since stem cells can proliferate and differentiate, their isolation facilitates transplantation and gene therapies as well as understanding the basic process of hematopoiesis. While progress has been made in the isolation of stem cells through biotin-avidin based immunoadsorption and fluorescence-activated cell sorting, improvements are still needed in order to increase yields, purity and rapidity of processing of stem cells. Recent advances by AmberGen, Inc. in the field of photocleavable reagents provide the basis of the proposed research. Photocleavable biotins allow specific cells to be separated from a heterogeneous population through the biotin-avidin interaction and then released by light. Photocleavable markers can be attached to cells through antibodies for cell sorting and then removed by light. Recent progress shows these photoreagents can be designed to photocleave at red-shifted wavelengths which are non-damaging to cells. Research will also be directed at the design and production of a novel class of antibodies whose interaction with antigens can be modulated by light. AmberGen, Inc. will synthesize and evaluate a variety of photocleavable biotins, photocleavable markers and light-activated amino acids for the isolation of stem cells.

* information listed above is at the time of submission.

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