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Rapid, reliable and repeatable platforms for cell-free prototyping

Award Information
Agency: Department of Defense
Branch: Defense Advanced Research Projects Agency
Contract: W911NF-16-P-0003
Agency Tracking Number: D152-001-0059
Amount: $149,999.91
Phase: Phase I
Program: SBIR
Solicitation Topic Code: SB152-001
Solicitation Number: 2015.2
Timeline
Solicitation Year: 2015
Award Year: 2016
Award Start Date (Proposal Award Date): 2015-10-08
Award End Date (Contract End Date): 2016-10-12
Small Business Information
1785 Beloit Ave Apt. 309
Los Angeles, CA 90025
United States
DUNS: 079761816
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Zachary Sun
 Co-founder, CEO
 (646) 725-6686
 zachary.sun@gmail.com
Business Contact
 Zachary Sun
Title: program manager
Phone: (646) 725-6686
Email: zachary.sun@gmail.com
Research Institution
N/A
Abstract

Cell-free platforms are a disruptive technology that can dramatically speed up the design-build-test cycle of biomolecular engineering. However, most currently available cell-free platforms are optimized for protein expression rather than for prototyping. Synvitrobio proposes to develop and commercialize a next-generation cell-free platform that allows for rapid, low-cost, high-throughput, and reproducible prototyping of complex biomolecular networks that emulate the in vivo environment. This next-generation cell-free platform is based on the published E. coli TX-TL system, which has been demonstrated to enable prototyping of complex circuits and pathways in vitro. Utilizing its experience and expertise in cell-free systems, Synvitrobio will contribute improvements in five key categories: cost, variability, scale, deployment, and diversity. Improvements will be tailored to specific cell-free applications of defense, commercial, and academic interest, and the cell-free platforms will be optimized against circuit behavior in vivo rather than purely against expression. We anticipate 4-100x fold cost-reductions, 4-50x variability reductions, and 4-100x scale increases over the current standard. We will also develop multiple distribution and cell-free platforms from different organisms to expand the application space of cell-free platforms.

* Information listed above is at the time of submission. *

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