MULTI-SAMPLE COMPARATIVE RT-PCR

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43CA096388-01
Agency Tracking Number: CA096388
Amount: $119,783.00
Phase: Phase I
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
AMBION, INC., 2130 WOODWARD ST, #200, AUSTIN, TX, 78746
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 DAVID BROWN
 (512) 651-0200
 DBROWN@AMBION.COM
Business Contact
 RIGO VALLEJO
Phone: (512) 651-0200
Email: RVALLEJO@AMBION.COM
Research Institution
N/A
Abstract
Ambion, Inc. proposes to adapt its Comparative RT-PCR procedure to accommodate the comparative analysis of single transcripts across one hundred RNA samples. The proposed procedure involves reverse transcribing a collection of RNA samples using primers with identical anchored oligodT and PCR primer binding sites but intervening hybridization domains that are unique to each sample. Equal amounts of the differentially tagged cDNAs are mixed and a specific target sequence in the sample mixture is amplified using a primer specific to the binding site present in the reverse transcription primer and a primer specific to the target of interest. A labeled nucleotide or primer is incorporated during PCR and the labeled products are hybridized to an array of probes specific to each of the sample-specific hybridization domains. The hybridization signal is quantified to reveal the relative abundance of the target in the RNA samples. The cDNA mixture can be used to evaluate many unique genes.

* Information listed above is at the time of submission. *

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