Genomic siRNA Libraries as Tools for Pseudo-Genetics

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$254,451.00
Award Year:
2003
Program:
SBIR
Phase:
Phase I
Contract:
1R43HG003270-01
Award Id:
65613
Agency Tracking Number:
HG003270
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
2130 WOODWARD STREET, AUSTIN, TX, 78744
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
DAVIDBROWN
(512) 651-0200
DBROWN@AMBION.COM
Business Contact:
RIGOVALLEJO
(512) 651-0200
RVALLEJO@AMBION.COM
Research Institute:
n/a
Abstract
DESCRIPTION (provided by applicant): The proposed research will enable mammalian cells to be used in mutation/selection experiments to identify genes involved in cellular processes. The objective of the proposal is to develop procedures for preparing siRNA libraries using genomic DNA and cDNA samples as the source material for siRNA templates. Libraries of siRNA vectors could comprise 1,000,000,000 to 1,000,000,000,000 unique siRNA expression vectors, making it possible to cover entire mammalian genomes with overlapping siRNAs. Mammalian cell populations transfected or transduced with siRNA vector libraries will be placed under selection to create cell populations expressing a desirable phenotype. The siRNA vector(s) integrated in the genomes of the selected cells will be amplified, cloned, and sequenced to reveal the siRNA template sequence. The siRNA sequences will be used to identify the genes whose down-regulation create the phenotype that was selected. The siRNA vector libraries should enable mutation/selection experiments to be used to correlate gene function and identity in mammalian cells.

* information listed above is at the time of submission.

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