Lateral Flow Immunoassay for Rapid Detection of Borrelia burgdorferi from Ticks

Award Information
Agency: Department of Defense
Branch: Defense Health Program
Contract: W81XWH-15-C-0163
Agency Tracking Number: H151-006-0012
Amount: $149,715.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: DHP15-006
Solicitation Number: 2015.1
Timeline
Solicitation Year: 2015
Award Year: 2015
Award Start Date (Proposal Award Date): 2015-09-29
Award End Date (Contract End Date): 2015-04-28
Small Business Information
1664 N. Virginia St., Applied Research Facility MS 328, Reno, NV, 89557
DUNS: 078634704
HUBZone Owned: Y
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Mark Hubbard, Ph.D.
 Chief Technology Officer
 (775) 223-6169
 mhubbard@dxdiscovery.com
Business Contact
 David Maine
Phone: (775) 223-6169
Email: dmaine@dxdiscovery.com
Research Institution
N/A
Abstract
Lyme disease is a vector-borne illness caused by infection with B. burgdorferi. Hard ticks (Ixodes spp.) acquire the spirochete from a natural reservoir of small mammals; transmission to humans occurs via the bite of an infected tick. By any measure, Lyme disease is one of the most serious emerging infectious diseases that threaten public health in the United States. New estimates from the CDC indicate that as many as 300,000 people are infected each year. Endemic areas of Lyme disease occur along the northeastern and Midwest regions of the United States. These areas, however, continue to spread and in 2013 forty-five of the fifty states reported at least one case. The goal of this SBIR proposal is to develop a field-deployable assay to rapidly detect B. burgdorferi in ticks. This technology will greatly increase the readiness of Service personnel and the general public against the threat of Lyme disease. Detection will be accomplished by constructing a lateral flow immunoassay (LFI) that targets the outer membrane lipoprotein OspA, which is abundantly expressed by B. burgdorferi within the tick midgut. The LFI is an ideal assay for resource-limited settings; key features include portability, high sensitivity/specificity, and rapid result (<30 min. inclusive of sample preparation).

* Information listed above is at the time of submission. *

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