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A rapid and high-throughput microfluidic stem cell analyzer

Award Information
Agency: Department of Defense
Branch: Defense Health Program
Contract: W81XWH-15-C-0112
Agency Tracking Number: H2-0091
Amount: $999,993.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: OSD11-H14
Solicitation Number: 2011.2
Timeline
Solicitation Year: 2011
Award Year: 2015
Award Start Date (Proposal Award Date): 2015-09-15
Award End Date (Contract End Date): 2018-02-20
Small Business Information
701 McMillian Way NW, Suite D, Huntsville, AL, 35806
DUNS: 000000000
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 Yi Wang
 Director
 (256) 726-4915
 proposals-contracts@cfdrc.com
Business Contact
 Deborah Phipps
Phone: (256) 726-4884
Email: dap@cfdrc.com
Research Institution
N/A
Abstract
Current methods for stem cell isolation are time-consuming, costly, and labor-intensive, and ill-suited for point of care applications. To overcome these limitations, we propose to develop and demonstrate a high-throughput, non-invasive, microfluidic stem cell analyzer to enable a rapid isolation of high-quality stem cell products from clinically relevant samples. Our technology enables significant improvements in processing time, automation, cell integrity, and logistical burden and cost, and opens a new possibility for interaoperative therapeutics. In the initial effort, key technology components were successfully developed. Device design, microfabrication, and experimental test were all undertaken to demonstrate microfluidic cell sorting and impedance characterization of stem cell differentiation, which markedly enhances TRL of the technology. This proposed effort will focus along two directions. First, a novel cell sorter will be developed to address challenges associated with clinical samples, such as large volume, high cell contents, target cell rarity, etc. Design optimization, fabrication refinement, and experimental characterization will be carried out to establish feasibility of the non-culture based stem cell isolation. Second, the sorter will be integrated with COTS component technologies for automated operation to improve GMP-readiness of the technology. The functionality of stem cell isolation will be extensively demonstrated using various pre-clinical samples, e.g., bone marrow, adipose, skin etc. A multi-disciplinary team with experience in all aspects of the proposed effort including microfluidics, stem cell bioengineering, regenerative medicine, and systems engineering has been assembled to ensure successful completion of project milestones.

* Information listed above is at the time of submission. *

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