Nonradioactive Receptor Binding Assays

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$76,286.00
Award Year:
1995
Program:
SBIR
Phase:
Phase I
Contract:
1 R43 MH53461-01,
Award Id:
29586
Agency Tracking Number:
29586
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Cornell Business Park 701-4, Wilmington, DE, 19801
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Charles Saller
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
The use of biotin labeled ligands in receptor binding assays will be used as a substitute for radioHigh affinity biotinylated ligands are used in immunochemistry studies but are not generally employebinding experiments. Biotinylated ligands also have high affinities for the proteins avidin and streproteins can be coupled with various enzymes which catalyze spectrophotometric, fluorometric, or chedetection in microliter plates. Using standard receptor binding protocols, biotinylated ligands canappropriate receptor and possible competitors for the receptor. Free or receptor bound biotinylatedby binding to the avidin or st reptavidin enzyme conjugates. This mechanism makes it possible for onbiotinylated ligand to be responsible for the generation of many molecules of product, providing aninitial signal. Several strategies for selected receptor binding assays, utilizing biotinylated ligaassay format assays will be examined. The knowledge gained in these investigations should aid in theadditional high throughput nonradioactive receptor binding assays.

* information listed above is at the time of submission.

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