Nonradioactive Receptor Binding Assays

Award Information
Agency:
Department of Health and Human Services
Amount:
$76,286.00
Program:
SBIR
Contract:
1 R43 MH53461-01,
Solitcitation Year:
N/A
Solicitation Number:
N/A
Branch:
N/A
Award Year:
1995
Phase:
Phase I
Agency Tracking Number:
29586
Solicitation Topic Code:
N/A
Small Business Information
Analytical Biological
Cornell Business Park 701-4, Wilmington, DE, 19801
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
N/A
Principal Investigator
 Charles Saller
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
The use of biotin labeled ligands in receptor binding assays will be used as a substitute for radioHigh affinity biotinylated ligands are used in immunochemistry studies but are not generally employebinding experiments. Biotinylated ligands also have high affinities for the proteins avidin and streproteins can be coupled with various enzymes which catalyze spectrophotometric, fluorometric, or chedetection in microliter plates. Using standard receptor binding protocols, biotinylated ligands canappropriate receptor and possible competitors for the receptor. Free or receptor bound biotinylatedby binding to the avidin or st reptavidin enzyme conjugates. This mechanism makes it possible for onbiotinylated ligand to be responsible for the generation of many molecules of product, providing aninitial signal. Several strategies for selected receptor binding assays, utilizing biotinylated ligaassay format assays will be examined. The knowledge gained in these investigations should aid in theadditional high throughput nonradioactive receptor binding assays.

* information listed above is at the time of submission.

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