High Resolution Multispectral Flow Imaging of Cells with Extended Depth of Field

Award Information
Agency: National Aeronautics and Space Administration
Branch: N/A
Contract: NNA04CB23C
Agency Tracking Number: 033451
Amount: $69,999.00
Phase: Phase I
Program: SBIR
Awards Year: 2004
Solicitation Year: 2003
Solicitation Topic Code: B2.02
Solicitation Number: N/A
Small Business Information
Amnis Corporation
2505 Third Avenue, Suite 210, Seattle, WA, 98121-1480
DUNS: 142155196
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 David Basiji
 Principal Investigator
 (206) 374-7165
 basiji@amnis.com
Business Contact
 Ralph Jorgenson
Title: Business Official
Phone: (206) 576-6857
Email: ralph@amnis.com
Research Institution
N/A
Abstract
Proposed is the development the extended depth of field (EDF) or confocal like imaging capabilities of a breakthrough multispectral high resolution imaging flow cytometer. This platform shall have unparalleled cellular analysis capabilities intended to further biological space research (fundamental, micro-gravity and radiation biology) and the potential capability of monitoring astronaut health. The proposed platform shall simultaneously combine the high throughput analysis rates of flow cytometry, the high resolution imaging capabilities of multiple forms of high resolution microscopy (brightfield, darkfield and four fluorescent imaging channels) and the ability to image all cellular components in focus utilizing extended depth of field imaging. This cell observation platform shall find additional utility in NASA?s biology space research given Amnis? complimentary technologies, specifically: i) Amnis? in-suspension labeling techniques for staining cellular structures and probing specific molecules in the nucleus, cytoplasm and membrane including fluorescent in situ hybridization. These in-suspension techniques eliminate the time consuming manual glass microscope slide preparation of cells which is problematic for micro gravity environment, ii) Amnis? sample containment/injection pump operates similarly to NASA?s rotating wall culture vessel allowing cells to be continually suspended via a rotational axis perpendicular to gravity.

* information listed above is at the time of submission.

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