High Resolution Multispectral Flow Imaging of Cells with Extended Depth of Field

Award Information
Agency:
National Aeronautics and Space Administration
Branch
n/a
Amount:
$69,999.00
Award Year:
2004
Program:
SBIR
Phase:
Phase I
Contract:
NNA04CB23C
Agency Tracking Number:
033451
Solicitation Year:
2003
Solicitation Topic Code:
B2.02
Solicitation Number:
n/a
Small Business Information
Amnis Corporation
2505 Third Avenue, Suite 210, Seattle, WA, 98121-1480
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
142155196
Principal Investigator:
David Basiji
Principal Investigator
(206) 374-7165
basiji@amnis.com
Business Contact:
Ralph Jorgenson
Business Official
(206) 576-6857
ralph@amnis.com
Research Institution:
n/a
Abstract
Proposed is the development the extended depth of field (EDF) or confocal like imaging capabilities of a breakthrough multispectral high resolution imaging flow cytometer. This platform shall have unparalleled cellular analysis capabilities intended to further biological space research (fundamental, micro-gravity and radiation biology) and the potential capability of monitoring astronaut health. The proposed platform shall simultaneously combine the high throughput analysis rates of flow cytometry, the high resolution imaging capabilities of multiple forms of high resolution microscopy (brightfield, darkfield and four fluorescent imaging channels) and the ability to image all cellular components in focus utilizing extended depth of field imaging. This cell observation platform shall find additional utility in NASA?s biology space research given Amnis? complimentary technologies, specifically: i) Amnis? in-suspension labeling techniques for staining cellular structures and probing specific molecules in the nucleus, cytoplasm and membrane including fluorescent in situ hybridization. These in-suspension techniques eliminate the time consuming manual glass microscope slide preparation of cells which is problematic for micro gravity environment, ii) Amnis? sample containment/injection pump operates similarly to NASA?s rotating wall culture vessel allowing cells to be continually suspended via a rotational axis perpendicular to gravity.

* information listed above is at the time of submission.

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