NOVEL DETERGENTS FOR CRYSTALLIZING MEMBRANE PROTEINS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$431,183.00
Award Year:
1992
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
13798
Agency Tracking Number:
13798
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1280 Dussel Dr, Maumee, OH, 43537
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Melvin Keyes
(419) 891-3030
Business Contact:
() -
Research Institution:
n/a
Abstract
THE UNDERSTANDING OF PROTEIN FUNCTION IS GREATLY ENHANCED BYTHE AVAILABILITY OF THREE-DIMENSIONAL STRUCTURAL INFORMATIONOBTAINED BY X-RAY CRYSTALLOGRAPHY. THIS IS TRUE FOR MEMBRANE AS WELL AS WATER-SOLUBLE PROTEINS, BUT THE LARGE, WELL-ORDERED THREE-DIMENSIONAL CRYSTALS REQUIRED FOR X-RAY ANALYSES HAVE BEEN DIFFICULT TO PRODUCE IN THE CASE OF MEMBRANE PROTEINS, AS THEY TEND MORE NATURALLY TO FORM TWO-DIMENSIONAL ARRAYS. A FURTHER COMPLICATING FACTOR IN DEALING WITH MEMBRANE PROTEINS IS THE REQUIREMENT FOR DETERGENTS TO SOLUBILIZE ANDSTABILIZE THE NATIVE STRUCTURE. OF ALL THE DETERGENTS AVAILABLE AND TESTED, ONLY TWO (OCTYLGLUCOSIDE AND LDAO) HAVE PROVEN EFFECTIVE FOR GROWING CRYSTALS SUITABLE FOR HIGH-RESOLUTION X-RAY ANALYSIS. ALTHOUGH CRYSTALLIZATION OFMEMBRANE PROTEINS IS MORE OF AN ART THAN A SCIENCE AS YET, IT IS CLEAR THAT THE STRUCTURE AND THE PURITY OF THE DETERGENT ARE CRITICAL FACTORS IN THE PROCESS. THIS PROJECT WILL SYNTHESIZE A HOMOLOGOUS SERIES OF NOVEL SUBSTITUTED GLUCOSIDES AND MALTOSIDES THAT WILL BE PURE, MILD (NONDENATURING), AND HAVE SOMEWHAT RIGID HYDROPHOBIC TAILS. THEY WILL BE EVALUATED FOR THEIR ABILITY TO SOLUBILIZE MEMBRANE PROTEINS IN AN ACTIVE, MONODISPERSE, STABLE FORM THAT MAY BE COMPATIBLE WITH THREE-DIMENSIONAL CRYSTAL GROWTH.

* information listed above is at the time of submission.

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