Mulitplexed protein measurement by real-time immuno-PCR

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,000.00
Award Year:
2004
Program:
SBIR
Phase:
Phase I
Contract:
1R43HG003508-01
Award Id:
70878
Agency Tracking Number:
HG003508
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
QUALITY PROTEOMICS, LLC (Currently AMPLIFIED PROTEOMICS, INC.)
QUALITY PROTEOMICS, LLC, 2462 WYANDOTTE ST, MOUNTAIN VIEW, CA, 94043
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
DAVID WILSON
(510) 432-0489
DSCOWILSON@YAHOO.COM
Business Contact:
JACK ZHAI
(510) 866-5515
JYZHAI@YAHOO.COM
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): The real-time polymerase chain reaction (PCR) is the most commonly used method for quantitative gene expression profiling; it allows extremely sensitive measurement of hundreds of mRNA transcripts in parallel. We propose a method by which protein abundances can be quantitated using the same technology. This is achieved by implementing a sandwich immuno-assay format in which the detection antibodies are labeled with different DNA sequences. Real-time PCR is then used to quantify all of the DNA labels in parallel, thus revealing the quantities of the cognate proteins in the biological samples. Preliminary experiments show that real-time PCR using such antibody-DNA conjugates gives over two orders of magnitude more sensitive detection and almost three orders of magnitude increased dynamic range in comparison to standard ELISA, and standard deviations are very low (CV approximately 5%). Initial evidence supporting the ability to multiplex this method has also been obtained.

* information listed above is at the time of submission.

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