A METHIONINASE FOR METHIONINE-DEPENDENT CHEMOTHERAPY

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 7118
Amount: $486,774.00
Phase: Phase II
Program: SBIR
Awards Year: 1990
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Anticancer Inc.
5325 Metro St, San Diego, CA, 92110
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Peter H Stern Phd
 (619) 299-3250
Business Contact
Phone: () -
Research Institution
N/A
Abstract
NEW APPROACHES ARE NECESSARY TO IMPROVE CHEMOTHERAPY OF SOLID TUMORS. FOR THIS PURPOSE, ANTICANCER, INC. HAS TAKEN ADVANTAGE OF THE CANCER-SPECIFIC METABOLIC DEFECT OF METHIONINE DEPENDENCE, WHICH CAUSES MANY TYPES OF CANCER CELLS TO REVERSIBLY ARREST LATE IN THE S/G2 PHASE OF THE CELL CYCLE IN MEDIUM WHERE METHIONINE (MET) HAS BEEN REPLACED BY HOMOCYSTEINE (HCY) (MET-HCY+ MEDIUM). THIS APPROACH HAS BEEN TERMED "METHIONINE-DEPENDENT CHEMOTHERAPY". TO BRING METHIONINE-DEPENDENT CHEMOTHERAPY CLOSER TO CLINICAL TRIALS, ANTICANCER, INC. PROPOSES TO ISOLATE A METHIONINASE THAT, UPON ADMINISTRATION TO THE ANIMAL OR PATIENT, WILL ALLOW THE MODULATION OF CIRCULATING LEVELS OF METHIONINE. THE BACTERIA ALCALIGENES FAECALIS AND PSEUDOMONAS PSEUDOALCALIGENES, WHICH CONTAIN A METHIONINASE THAT APPARENTLY DOES NOT CLEAVE HOMOCYSTEINE, HAVE BEEN IDENTIFIED. STATE-OF-THE-ART GENETIC SELECTION AND PROTEIN PURIFICATION PROCEDURES WILL BE USED TO ISOLATE A METHIONINASE WITH A LOW KM, A LONG HALF-LIFE IN THE CIRCULATION, AND A LOW TOXICITY FOR THE EXPERIMENTAL ANIMAL OR HUMAN PATIENT. THE METHIONINE-CLEAVING PROPERTIES, HALF-LIFE, AND TOXICITY OF THE ENZYME WILL BE TESTED IN RATS. PURIFICATION AND CHARACTERIZATION OF A DESIRABLE ENZYME IN PHASE I WILL LEAD TO CLONING THE METHIONINASE GENEIN PHASE II.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government