HIV LI-KEY/PEPTIDE VACCINE

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$301,761.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
1R43AI051023-01
Award Id:
60453
Agency Tracking Number:
AI051023
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
ANTIGEN EXPRESS, INC., 100 BARBER AVE, WORCESTER, MA, 01606
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
MINZHEN XU
(508) 852-8783
ANTIGENEXP@AOL.COM
Business Contact:
ROBERT HUMPHREUYS
(508) 852-8801
ANTIGENEXP@AOL.COM
Research Institution:
n/a
Abstract
Antigen Express scientists have discovered that a segment of the Ii protein called Ii-Key, which acts at an allosteric site on one end of the antigenic peptide binding site on MHC Class II molecules, greatly enhances the binding of a tethered epitope. Linkage of such Ii-Key with a MHC class II-restricted epitope in a hybrid enhances the potency of presentation of the epitope >200 times, in vitro with murine and human assays, inducing a TH1 pattern of cytokine release. We propose to test such compounds with HIV MHC class II-restricted epitopes to expand CD4+ immunity and memory toward enhancing efficacy of HIV CTL peptide vaccines, the safest approach to HIV vaccination. We will: 1) design and synthesize several Ii-Key/HIV class II epitope hybrids according to our established methods; 2) test in mice the potencies of these hybrids in terms of T helper cell proliferation and the induction of neutralizing antibody against class II-restricted HIV epitope; and 3) design and synthesize an Ii-Key/class II epitope/CTL epitope double hybrid according to the data of 2); and then test the vaccine efficacy of this double hybrid in CTL induction. Our experiments will lead to a very potent HIV peptide vaccine. In a Phase II program the efficacy of such hybrids will be tested in higher primates and man.

* information listed above is at the time of submission.

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