HIV LI-KEY/PEPTIDE VACCINE

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43AI051023-01
Agency Tracking Number: AI051023
Amount: $301,761.00
Phase: Phase I
Program: SBIR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
ANTIGEN EXPRESS, INC.
ANTIGEN EXPRESS, INC., 100 BARBER AVE, WORCESTER, MA, 01606
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 MINZHEN XU
 (508) 852-8783
 ANTIGENEXP@AOL.COM
Business Contact
 ROBERT HUMPHREUYS
Phone: (508) 852-8801
Email: ANTIGENEXP@AOL.COM
Research Institution
N/A
Abstract
Antigen Express scientists have discovered that a segment of the Ii protein called Ii-Key, which acts at an allosteric site on one end of the antigenic peptide binding site on MHC Class II molecules, greatly enhances the binding of a tethered epitope. Linkage of such Ii-Key with a MHC class II-restricted epitope in a hybrid enhances the potency of presentation of the epitope >200 times, in vitro with murine and human assays, inducing a TH1 pattern of cytokine release. We propose to test such compounds with HIV MHC class II-restricted epitopes to expand CD4+ immunity and memory toward enhancing efficacy of HIV CTL peptide vaccines, the safest approach to HIV vaccination. We will: 1) design and synthesize several Ii-Key/HIV class II epitope hybrids according to our established methods; 2) test in mice the potencies of these hybrids in terms of T helper cell proliferation and the induction of neutralizing antibody against class II-restricted HIV epitope; and 3) design and synthesize an Ii-Key/class II epitope/CTL epitope double hybrid according to the data of 2); and then test the vaccine efficacy of this double hybrid in CTL induction. Our experiments will lead to a very potent HIV peptide vaccine. In a Phase II program the efficacy of such hybrids will be tested in higher primates and man.

* information listed above is at the time of submission.

Agency Micro-sites

US Flag An Official Website of the United States Government