INDICATOR CELLS FOR ANTIVIRAL SCREENING FOR FILOVIRUSES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,000.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
1R43AI052917-01
Award Id:
60520
Agency Tracking Number:
AI052917
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
893 N. WARSON RD., ST. LOUIS, MO, 63141
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
PAUL OLIVO
(314) 812-8144
OLIVO@APATH.COM
Business Contact:
L MILTON
(314) 812-8160
MILTON@APATH.COM
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): The goal of this proposal is to develop the commercial potential of a cell-based assay to screen for compounds with antiviral activity against filoviruses such as Ebola and Marburg viruses. The basis of this bioassay, which has been prototyped with respiratory syncytial virus, is infection-independent expression of a reporter gene from an artificial viral genome present within the cytoplasm of transformed cells. Filoviruses include some of the most notorious human pathogens and cause significant morbidity and mortality due to severe hemorrhagic fever syndromes. Unfortunately, very few therapeutic agents are available to treat infections with these viruses. Efforts to find new antiviral agents will require methods that are amenable to highthroughput assays. Since all viruses are obligate intracellular microbes, any bioassay that determines the effect of various agents on viral replication, must be based on cell culture. This involves working with infectious virus, which is obviously not feasible for agents such as EBOV and MBGV. Significant advances have been made in identifying the cis-acting elements and trans-acting factors involved in replication of many pathogenic negative-strand RNA viruses, including these filoviruses. We plan to develop infection-independent Ebola and Marburg virus minigenome-expression systems in which the expression of an easily measurable enzyme is dependent on the RNA replication and transcription factors that are essential for viral replication. We plan to use these systems in cell-based assays for screening antiviral compounds.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government