A Novel Approach for Real-time Proteomics in Live Cells

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$1,130,450.00
Award Year:
2010
Program:
SBIR
Phase:
Phase II
Contract:
2R44RR024325-03
Agency Tracking Number:
RR024325
Solicitation Year:
2010
Solicitation Topic Code:
NCRR
Solicitation Number:
PHS2010-2
Small Business Information
POWERHOUSE PROTEOMIC SYSTEMS, LLC
1623 CATALINA WAY, ZIONSVILLE, IN, -
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
602491495
Principal Investigator:
VAIDYANATHAN SUBRAMANIAM
() -
VAIDYA@POWERPROT.COM
Business Contact:
VAIDYANATHAN SUBRAMANIAM
(317) 640-2266
vaidya@powerprot.com
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): The ultimate goal of this SBIR project is to develop a novel proteomic system that will enable the real- time expression profiling of proteins in live murine embryonic stem (mES) cells and in differentiated cells derived there from. The first step involves the establishment of a library of fluorescent trap mES cell lines, each of which will harbor a single fluorescently-tagged protein. The fluorescence in each cell of a particular clonal line will serve as a reporter for the expression and subcellular localization of the tagged protein in that cell line. Such a library of clonal fluorescently-tagged lines can be subjected to quantitative fluorescence microscopy in multiwell live-cell arrays, to enable the simultaneous real-time expression profiling of multiple proteins in live cells under various culture conditions. In Phase I we demonstrated the feasibility of establishing a large and diverse protein-trap library using our approach. This proposed Phase II effort will be focused generating a library of at 1500 unique fluorescent protein-trap mES cell lines. Individual cell lines from such a library of fluorescently protein-trap mES lines can also be used as versatile protein-specific research tools as they can be manipulated in culture into different tissue-types or used for the generation of transgenic mice when desired. Many also these cell lines can also be used to design novel powerful cell-based assays. Therefore this proposed Phase II project will result in the generation of a versatile and diverse proteomic research resource in mES cells. PUBLIC HEALTH RELEVANCE: The ultimate aim of this SBIR project is to develop a system that will enable researchers to monitor the location and levels of hundreds of proteins simultaneously in living cells. Such a system will be useful in researching a wide variety of biological processes, including understanding the molecular basis of a number of human diseases. Further, such a system can be applied to the identification therapeutic targets and the creation of powerful drug-screening platforms to identify potential therapeutic agents that may cure these diseases.

* information listed above is at the time of submission.

Agency Micro-sites

US Flag An Official Website of the United States Government