Fluorophore Labeled Nucleic Acid Probes

Award Information
Agency:
Department of Health and Human Services
Amount:
$75,000.00
Program:
SBIR
Contract:
1 R43 CA63991-1,
Solitcitation Year:
N/A
Solicitation Number:
N/A
Branch:
N/A
Award Year:
1994
Phase:
Phase I
Agency Tracking Number:
24877
Solicitation Topic Code:
N/A
Small Business Information
Aphios Corp.
3-E Gill Street, Woburn, MA, 01801
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
N/A
Principal Investigator
 Salifu Dakubu
 (617) 932-6933
Business Contact
Phone: () -
Research Institution
N/A
Abstract
This investigation is aimed at devising ways and special instrumentation for using fluorescentlanthanide chelates, detected by time resolved fluorometry, as a means for detecting nucleic acids in avariety of settings such as Southern blots and cells or tissues (e.g., as in situ hybridization). Thesemethodologies have the potential of high sensitivity, since time-resolved fluorometry permits detectionof signal in the virtual absence of background fluorescence. Therefore, high intensity light can be usedto excite the fluorescence. Established techniques and literature indicate that for other applications thistype of approach is at least as sensitive as other methods, including radioisotopic or chemiluminescence--based or enzyme-based methods. This approach would have the advantage of speed, since aphotographic or electronic imaging record of the results can be obtained essentially instantaneously.

* information listed above is at the time of submission.

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