Mouse Chromosome-Specific Painting Probes

Award Information
Agency:
Department of Health and Human Services
Amount:
$735,111.00
Program:
SBIR
Contract:
N/A
Solitcitation Year:
N/A
Solicitation Number:
N/A
Branch:
N/A
Award Year:
1995
Phase:
Phase II
Agency Tracking Number:
22291
Solicitation Topic Code:
N/A
Small Business Information
Applied Genetics
1335 Gateway Dr., Melbourne, FL, 32901
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
N/A
Principal Investigator
 Melissa C. Liechty
 (407) 768-2048
Business Contact
Phone: () -
Research Institution
N/A
Abstract
We have described a new technology called "preparative in situ hybridization (Prep-I.S.H.), which is capable of isolating a set of cDNA or genomic clones according to their chromosome location. The technology takes advantage of in situ hybridization of cloned DNAs to metaphase chromosome spreads, chromosome microdissection, and the polymerase chain reaction (PCR). We will use this technology to develop a set of chromosome-specific painting probes for the mouse. The demonstration case, a chromosome 11-specific probe, will be used to paint the mitotic chromosome 11s derived from the mouse lymphoma assay (MLA).

* information listed above is at the time of submission.

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