Novel immune stimulating adjuvants for an HIV DNA Vaccine

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$283,473.00
Award Year:
2010
Program:
SBIR
Phase:
Phase I
Contract:
1R43AI089288-01
Award Id:
95787
Agency Tracking Number:
AI089288
Solicitation Year:
n/a
Solicitation Topic Code:
NIAID
Solicitation Number:
n/a
Small Business Information
TECHCENTER@UMBC, 1450 SOUTH ROLLING RD., BALTIMORE, MD, 21227
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
185576639
Principal Investigator:
KENNETH BAGLEY
() -
BAGLEY@PROFECTUSBIOSCIENCES.COM
Business Contact:
JEFFREY MESHULAM
() -
meshulam@profectusbiosciences.com
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): This document contains proprietary information that Profectus BioSciences requests not be released to persons outside the Government, except for purposes of review and evaluation. Abstract The enhanced safety, stability , and accelerated product development generally provided by DNA vaccination make it an appealing approach to develop prophylactic and therapeutic HIV vaccines. Unfortunately, successful DNA vaccination of primates requires multiple inoculations with undesi rably large doses of plasmid. Genetically encoded adjuvants could provide the immune stimulating and dose-sparing effects necessary to realize a practical DNA-based vaccine for HIV. Our adjuvant approach exploits the RIG-1 signaling cascades that recognize infection with viral pathogens and trigger innate immune responses. We have constructed dominant-positive versions of RIG-1-like helicases and their adaptor protein (IPS-1) that mimic viral infection when transfected into cells as plasmid DNA. Our objecti ve is to determine whether these plasmid expressed RIG-1 based constructs will adjuvant an HIV DNA vaccine in mice to a greater extent than the bench mark adjuvant IL-12. We will pursue our objective through the following specific aims: (1) Identify an a djuvant or adjuvant combination that induces maximal B and T cell mediated immune responses against HIV GP160; (2) Demonstrate that the lead adjuvant provides enhanced protection in a Vaccinia-HIV challenge model. The co-formulation that is immunogenic and protective in mice will be evaluated in additional mice and primate studies as components of an advanced HIV DNA vaccine in follow-on Phase II SBIR applications. 2 PUBLIC HEALTH RELEVANCE: The objective of this project is to develop novel adjuvants for an HIV DNA vaccine. Such a vaccine/adjuvant combination is needed to combat the HIV epidemic.

* information listed above is at the time of submission.

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