DNA Purification Form Single Cells

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 N43 DK52223-000,
Agency Tracking Number: 29411
Amount: $98,790.00
Phase: Phase I
Program: SBIR
Awards Year: 1995
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Applied Imaging Corp.
2380-B Walsh Avenue, Santa Clara, CA, 95051
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Alex Saunders
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
Long term objective is to purify DNA of fetal origin, starting with a sample of maternal blood. Thtechnology to be used with a variety of Diagnostic Methods including Polymerase Chain Reaction (PCR)Genomic Hybridization (CGH), Electrophoresis. The whole human genome becomes analyzable for a fetuswithout invading the fetus for a sample. Steps already available at Applied Imaging include enrichmeautomated microscope. Operator editing of the cells found ensures that only fetal cells are used.light activated protective layer to stabilize the single cells of interest, while eliminating all otwill also design the means to remove the light activated protective layer and make the DNA from thesfor further processing. Phase II will join the Phase I methods with an amplification system (such awhole DNA of fetal cells is made available for further study. This will be joined to existing methoelectrophoresis, to demonstrate usefulness of the proposed procedure.

* information listed above is at the time of submission.

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