DEVELOPMENT OF PROTEASE-DEFICIENT, GRAM-POSITIVE HOST

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$252,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
4685
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Applied Microbiology Inc.
#5 Brooklyn Navy Yard, Brooklyn, NY, 11205
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Peter Blackburn
Principal Investigator
(718) 852-5353
Business Contact:
() -
Research Institution:
n/a
Abstract
OUR LONG-TERM AIM IS TO DEVELOP A GRAM-POSITIVE ORGANISM FORTHE EXPRESSION AND SECRETION OF MEDICALLY AND INDUSTRIALLY USEFUL PROTEINS. ONE MAJOR OBSTACLE TO THE SUCCESSFUL DEVELOPMENT OF SUCH A SYSTEM IS THE PROTEOLYTIC DEGRADATION OF SECRETED PRODUCTS. SITE-DIRECTED DELETION MUTATIONS CONSTRUCTED IN VITRO HAVE ELIMINATED THE TWO MAJOR EXTRACELLULAR PROTEASES OF B. SUBTILIS. ALTHOUGH SIGNIFICANTLY DIMINISHED, PROTEOLYTIC DEGRADATION OF SECRETED PRODUCTS BY RESIDUAL PROTEASE REMAINS PROBLEMATIC. CHARACTERIZATION OF RESIDUAL PROTEASE ACTIVITY WILL BE PERFORMED PRELIMINARY TO CLONING AND SITE-DIRECTED DELETION OF ANY PROTEASE GENES IDENTIFIED IN THE PRESENT STUDY. MUTAGENESIS OF DOUBLE-PROTEASE-NEGATIVE STRAINS OF B. SUBTILIS, AND SCREENS OF OTHER BACILLUS SPECIES FOR PROTEASE-DEFICIENT STRAINS, WILL BE PERFORMED IN AN EFFORT TO DEVELOP A GRAM-POSITIVE HOST THAT DOES NOT DEGRADE SECRETORY PRODUCTS.

* information listed above is at the time of submission.

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