Characterization of Factor VII From Transgenic Fish

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 1R43HL068435-01
Amount: $98,016.00
Phase: Phase I
Program: SBIR
Awards Year: 2001
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 () -
Business Contact
Phone: (386) 418-1400
Research Institution
DESCRIPTION (provided by applicant): Organ specific expression of recombinant proteins in transgenic fish will be examined as an alternative to the production of complex proteins in tissue culture cells or the milk of large domestic animals. Fish have a high reproductive capacity, short breeding cycles, and embryos that are easy to manipulate. The fish bioreactor system will be appropriate for quick production of small quantities of protein for pre-clinical evaluation, and scale up to commercial production, at substantially lower costs than the current methods. Human coagulation Factor VII was chosen to test liver specific expression in fish. The recombinant protein will be purified and analyzed for specific activity, extent of gamma carboxylation of glutamic acid residues, and the degree of glycosylation. The expression level, purity and the cost per unit (or microgram) of the enzyme produced in fish can then be compared to those values for Factor VII products from other sources. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government