A Catalytic Conformational Prion Sensor

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 2R44HL070399-02
Agency Tracking Number: HL070399
Amount: $749,995.00
Phase: Phase II
Program: SBIR
Awards Year: 2003
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
ARETE ASSOCIATES
ARETE ASSOCIATES, 1725 JEFFERSON DAVIS HWY, ARLINGTON, VA, 22202
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 CINDY ORSER
 (301) 424-8344
 CORSER@ADLYFE.COM
Business Contact
 PHILIP SELWYN
Phone: (703) 413-0290
Email: SELWYN@ARETE-DC.COM
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): The major objective of the proposed project is to develop methodology that will allow fast and efficient detection of infectious prion proteins prior to the onset of clinical symptoms. This includes the detection of infectious prions in mortal products, early ante-mortem diagnostic of the prion diseases, and developing experimental approaches allowing delineation of the molecular mechanism of prion and prion-related diseases (folding disorders) in thermodynamic and kinetic terms. Preliminary studies successfully demonstrated that the specific detection of the pathologic prion protein could be approached by simple experimental means. The prion disease process involves a conformational change in the prion protein that in turn can serve as the basis for an early detection, diagnostic, prognostic and screening assay. The assay detects infectivity in a crude sample by identifying existing sub-picomolar levels of infectious prion protein through the use of specific, fluorescent target peptides. The labeled peptides undergo conformational change resulting in an amplified signal in a single step without protease pre-treatment, denaturants or washing. This methodology has three applications: first as a detection assay for infectious prion protein, second as a possible tool for similar diagnostics in other amyloid and/or mis-folded protein diseases; and, lastly as a tool for gaining a better understanding of the molecular mechanism of this group of diseases which can be used to optimize diagnostics and future therapeutics. The structural complexity of the target peptide sequence will be optimized and the thermodynamic and kinetic facets of peptide interaction with the infectious prion protein will be studied thoroughly at the quantitative level to highlight the prominent features that determine species specificity and to generate calibration curves with binding constants for diagnostic development. To further assay improvement, a predictive model will be developed for the conformational diversity of the target peptides used as indicators and surrogates of conformational change upon interaction with the infectious prion protein. The universality of the target peptide concept as a detector for other amyloid diseases will also be evaluated.

* information listed above is at the time of submission.

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