ISOLATION AND EXPRESSION OF CDNA CLONES FOR RIF

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$49,233.00
Award Year:
1991
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
16381
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Argus Pharmaceuticals, Inc
1515 Holcombe Blvd, Box 41, Houston, TX, 77030
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Tucker, Stanley D
Principal Investigator
(713) 367-1666
Business Contact:
1 R43 CA53040-01A1
() -
Research Institution:
n/a
Abstract
CYTOTOXIC ACTIVATED MACROPHAGES SECRETE SOLUBLE FACTORS THAT HAVE A VARIETY OF EFFECTS ON TUMOR CELLS. INCLUDED IN THE TUMOR CELL RESPONSE ARE THE INHIBITION OF MITOCHONDRIAL RESPIRATION AND INHIBITION OF DNA SYNTHESIS. A MONOKINE CALLED RIF, WHICH WAS FOUND TO REPRODUCE THESE EFFECTS IN VITRO, HAS BEEN CHARACTERIZED. THE ISOLATION AND VERIFICATION OF THE CDNA FOR RIF IS THE PRIMARY FOCUS OF THIS PROJECT. BASED ON THE SEQUENCE OBTAINED FROM THE 80KD PROTEIN PURIFIED IN THE RIF PREPARATIONS, A SYNTHETIC PEPTIDE WAS CONSTRUCTED AND ANTIBODIES AGAINST THE PEPTIDE WERE PRODUCED. THIS ANTIBODY PROBE AND AN ANTISERUM AGAINST A PREPARATION OF RIF PROTEIN WILL BE USED TO SCREEN A LAMBDA EXPRESSION LIBRARY IN ORDER TO ISOLATE A CDNA CLONE CORRESPONDING TO THE RIF PROTEIN. THE CDNA WILL BE FULLY CHARACTERIZED AND EXPRESSION STUDIES WILL BE INITIATED. THE AVAILABILITY OF RECOMBINANT PROTEIN AND CDNA CLONES WILL PERMIT A SECOND PHASE OF STUDIES WHICH WILL ENTAIL EXPRESSION STUDIES IN MAMMALIAN SYSTEMS, ANIMAL-TUMOR MODEL STUDIES OF RECOMBINANT PROTEINS, AND MECHANISM STUDIES OF THE RECOMBINANT PROTEIN.

* information listed above is at the time of submission.

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