DETECTING SERPULINA HYODYSENTERIAE- SPECIFIC DNA BY CHEMILUMINESCENCE

Award Information
Agency:
Department of Agriculture
Branch
n/a
Amount:
$49,820.00
Award Year:
1993
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
20939
Agency Tracking Number:
20939
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1430 E Ft Lowell #200, Tucson, AZ, 85719
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Ms Mary E Mapother
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
SWINE DYSENTERY (SD) HAS BEEN ESTIMATED TO COST SWINE PRODUCERS IN EXCESS OF $180 MILLION ANNUALLY, AND IS QUARANTINABLE IN MOST STATES. CONFIRMATION OF THE DISEASE IS BASED ON THE DETETION OF THE BACTERIA, SERPULINA HYODYSENTERIAE, FROM INFECTED PIGS. AN ACCURATE DIAGNOSIS OF SD CURRENTLY INCLUDES LABOR-INTENSIVE AND TIME CONSUMING PROCEDURES WHICH DO NOT ALWAYS RESULT IN THE DETECTION AND/OR ISOLATION OF THE CAUSATIVE AGENT. IN ADDITION, MANY VETERINARY DIAGNOSTIC LABORATORIES ACROSS THE COUNTRY ARE NOT SET-UP TO PERFORM ANAEROBIC BACTERIOLOGY, AND CONSEQUENTLY, MUST SEND SAMPLES TO MORE EXPERIENCED LABORATORIES FOR DIAGNOSIS. SINCE THERE ARE VERY FEW LABORATORIES IN THE U.S. WITH EXPERIENCE ISOLATING AND IDENTIFYING S. HYODYSENTERIAE FROM INFECTED PIGS, SAMPLES MAY NEED TO BE SENT GREAT DISTANCES AND RESULTS MAY NOT BE OBTAINED FOR MANY DAYS OR EVEN WEEKS. THE NEED FOR EASILY INTERPRETED, RAPID, AND SENSITIVE DIAGNOSTIC TESTS, WHICH REQUIRE NO SPECIALIZED LABORATORY EQUIPMENT, FOR SWINE DYSENTERY IS GREAT. WE PROPOSE THE DEVELOPMENT F A NUCLEIC ACID PROBE TEST, WHICH INCLUDES AN ENZYMATIC DETECTION SYSTEM, THAT COULD BE USED IN VIRTUALLY ANY DIAGNOSTIC SETTING FOR A FAST SENSITIVE, AND ACCURATE DIAGNOSIS OF THIS IMPORTANT DISEASE.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government