Parallel DNA Assembling on MicroChip

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R41HG003786-01A1
Agency Tracking Number: HG003786
Amount: $453,401.00
Phase: Phase I
Program: STTR
Awards Year: 2006
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
ATACTIC TECHNOLOGIES, INC.
2575 WEST BELLFORT, SUITE 270, HOUSTON, TX, 77054
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 XIAOLIAN GAO
 (713) 743-2805
 XGAO@UH.EDU
Business Contact
 XIAOCHUAN ZHOU
Phone: (713) 664-8189
Email: XCZHOU@ATACTICTECH.COM
Research Institution
 UNIVERSITY OF HOUSTON
 UNIVERSITY OF HOUSTON
4800 CALHOUN RD
HOUSTON, TX, 77204
 Nonprofit college or university
Abstract
DESCRIPTION (provided by applicant): The increasing use of DNA as a molecular tool has ushered the technology of de novo synthesis of DNA back to the center stage of modern biology. We propose the development of a robust, ultra high-throughput (UHT) technology capable of genome-scale production of high fidelity synthetic DNA at about one percent of the cost and time of the current technologies. Our phase I plan will demonstrate the feasibility of the proposed method that includes parallel synthesis of an array of long DNA sequences on a capture microchip using annealing and ligation of assembling deoxyoligonucleotides (oligos). Our Phase I Specific Aims are: 1: Developing a method for parallel synthesis of an array of long DNA sequences on chip; 2: Developing monitoring and error correction methods essential for improving the efficiency and fidelity of the DNA synthesis. The proposed solid phase parallel synthesis technology has two significant advantages over the existing methods of DNA synthesis: (i) it has the potential to allow simultaneous assembling of thousands or more DNA sequences; (ii) it is a process that individual steps and individual synthesis can be monitored and optimized. If successful, one can expect a significant step forward in long DNA sequence preparation, similar to the advancement by moving oligonucleotide and peptide synthesis from solution to solid phase. A reliable, miniaturized, robust process will then replace the current slow, expensive, and still largely unpredictable gene synthesis processes.

* information listed above is at the time of submission.

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government