Drug Target Discovery in Mast Cells by GECKO Technology

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43AI056636-01
Agency Tracking Number: AI056636
Amount: $99,800.00
Phase: Phase I
Program: SBIR
Awards Year: 2003
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 (216) 431-9900
Business Contact
Phone: (216) 431-9900
Research Institution
DESCRIPTION (provided by applicant): The goal of the present research program is to utilize a genome-wide, high-throughput gene knock-out technology in combination with cellular assays to identify anti-inflammatory drug targets. This novel knockout technology, termed Genome-wide Cell-based Knockout technology or GECKO, is capable of inactivating expression of each transcriptionally active gene in cultured cells. Genome-wide GECKO libraries contain a relatively small number of clones, typically less than 1,000,000 clones, wherein each clone contains a different gene knockout, and collectively each transcriptionally active gene has been knocked out within the library. In this phase I research program, we will establish mast cell activation assays compatible with the GECKO technology, and create a genome-wide GECKO library for identification of genes whose function is necessary for mast cell degranulation. The specific aims of this phase I program, therefore, are 1) to develop robust high-throughput assays to enable screens for mast cell mutants with defective activation and degranulation responses.; and 2) to generate a phenotypically stable, genome-wide, GECKO library for use in identifying degranulation mutants in the chosen mast cell model. Success in this Phase I program will enable a full scale effort to identify genes that inhibit various mast cell functions involved in allergy, asthma, and anaphylaxis as well as in degenerative fibrosis and arthritis, and thereby identify new drug targets for the treatment of inflammatory diseases.

* information listed above is at the time of submission.

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